Facile Structural Elucidation of Glycans Using NMR Spectroscopy Data and the Program CASPER : Application to the O-Antigen Polysaccharide of Escherichia coli O155

摘要

The program CASPER was successfully employed to rapidly elucidate a new O-antigen polysaccharide structure (obtained from a strain of Escherichia coli serogroup O155), using solelyunassigned NMR spectroscopy data as input information. Thus, what is considered the most tedious and time-consuming part of the structural elucidation process has been reduced from several hours (or even days) of manual interpretation to about four minutes of automated analysis.